The Open Microbiology Journal




ISSN: 1874-2858 ― Volume 13, 2019
RESEARCH ARTICLE

Molecular Study of Quinolone Resistance Determining Regions of gyrA Gene and parC Genes in Clinical Isolates of Acintobacter baumannii Resistant to Fluoroquinolone



Maysaa El Sayed Zaki*, Nermen Abou ElKheir, Mohamed Mofreh
Clinical Pathology Department, Mansoura Faculty of Medicine, Mansoura University, Mansoura, Egypt

Abstract

Introduction:

Acinetobacterb aumannii (A. baumannii) is an important pathogen in health care associated infections. Quinolone resistance has emerged in this pathogen.

Aims & Objectives:

The aim of the present study was to determine the presence of mutations of gyrA gene and parC genes by Restriction Fragment Length Polymorphism Polymerase Chain Reaction (RFLP-PCR) among clinical isolates of A. baumanii.

Materials and Methods:

The study was carried out on 140 clinical isolates of A. baumannii. The isolates were subjected to molecular study of mutations of gyrA gene and parC genes by RFLP–PCR beside determination of Minimal Inhibitory Concentration (MIC) by macro dilution tube method.

Results:

The isolates of A. baumannii were resistant to ciprofloxacine and levofloxacin at MIC >4 µg/ml. The most isolates had MIC >128 µg/ml (42.3%). All resistant strains to ciprofloxacin of A. baumannii had mutations in gyrA and parC. The most frequent mutations were combined mutations in both genes (85.5%) and 5% had single mutation either in gyrA or parC. The most frequently combined mutations were associated with MIC >128 µg/ml (42.3%).

Conclusion:

From this study we can conclude that resistance to ciprofloxacin was common in clinical isolates of A. baumannii. The most frequent mutations were present in gyrA and parC. However, mutations in parC alone were not uncommon. Further large scale studies are required to elucidate the resistance pattern of A. baumannii and its molecular mechanisms.

Keywords: A. baumannii, Quinolone resistance, RFLP-PCR, Molecular mechanisms, Clinical isolates, Mutations.


Article Information


Identifiers and Pagination:

Year: 2018
Volume: 12
First Page: 116
Last Page: 122
Publisher Id: TOMICROJ-12-116
DOI: 10.2174/1874285801812010116

Article History:

Received Date: 16/02/2018
Revision Received Date: 15/04/2018
Acceptance Date: 16/04/2018
Electronic publication date: 30/04/2018
Collection year: 2018

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© 2018 Zaki et al.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: (https://creativecommons.org/licenses/by/4.0/legalcode). This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


* Address correspondence to this author at the Clinical Pathology Department, Mansoura Faculty of Medicine, Mansoura University, Mansoura, Egypt; Tel: 01227185777; E-mail:may_s65@hotmail.com


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