The Open Microbiology Journal

ISSN: 1874-2858 ― Volume 13, 2019

Using Cellulolytic Nitrogen Fixing Bacterium, Azomonas agilis for Effective Degradation of Agricultural Residues

Zaw K. Latt1, San S. Yu1, Ei P. Kyaw1, Tin M. Lynn1, *, May T. Nwe1, Wai W. Mon1, Kyaw N. Aye2
1 Biotechnology Research Department, Ministry of Education, Kyaukse, Mandalay Division, 100301, Myanmar
2 Department of Chemical Engineering, Yangon Technological University, Yangon, Myanmar



Azomonas agilis, a nitrogen-fixing bacterium, was isolated from rhizospheric soil in central Myanmar.

Methods & Materials:

The nitrogen-fixing activity of this bacterium was detected by plate screening method using glucose nitrogen free mineral medium and ammonium test-kit Cellulolytic activity was screened by plat assay and detected by Dinitrosalicyclic acid method (DNS).

Results & Discussion:

The isolated A. agilis grew in media containing 3-12% of NaCl, although the growth became poor when NaCl concentrations increased. Among various carbon sources, sucrose was the best source for ammonium accumulation of this bacterium, whereas arabinose was not the suitable carbon source. Although the nitrogen-fixing activity of A. agilis was highest after one week incubation, cellulase enzyme production was highest after 2-3 days of incubation. It was observed that cellulase enzyme activity of A. agilis for cellulose and sodium carboxymethyl cellulose (CMC) was almost the same. Three agricultural wastes were used to detect the cellulase enzyme activity of A. agilis, cellulase activity was better on filter paper as a substrate when compared to rice-straw and sawdust.


So, the isolated A. agilis has high potential as an effective bacterial strain to use in sustainable agriculture and degradation of some agricultural residues.

Keywords: Azomonas agilis, Nitrogen fixation, Cellulose, NaCl, Reducing sugar , Sustainable agriculture .

Article Information

Identifiers and Pagination:

Year: 2018
Volume: 12
First Page: 154
Last Page: 162
Publisher Id: TOMICROJ-12-154
DOI: 10.2174/1874285801812010154

Article History:

Received Date: 15/2/2018
Revision Received Date: 07/05/2018
Acceptance Date: 10/05/2018
Electronic publication date: 31/05/2018
Collection year: 2018

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© 2018 Latt et al.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: ( This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

* Address correspondence to the author at the Biotechnology Research Department, Ministry of Education, Kyaukse, Myanmar, Tel: +95-9-975985633, Fax: +95-66-50622; E-mail:


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