The Open Microbiology Journal




ISSN: 1874-2858 ― Volume 12, 2018
RESEARCH ARTICLE

Characterization of Actinobacterial Communities from Arauca River Sediments (Colombia) Reveals Antimicrobial Potential Presented in Low Abundant Isolates



Carolina Arango1, Alejandro Acosta-Gonzalez1, Claudia M. Parra-Giraldo2, Zilpa A. Sánchez-Quitian2, Russell Kerr3, Luis E. Díaz1, *
1 Universidad de La Sabana, Campus del Puente del Común, Km 7 Autopista Norte de Bogotá, Chia, Colombia
2 Unidad de Investigación en Proteómica y Micosis Humanas, Pontificia Universidad Javeriana, Carrera 7 No. 40-62, Bogotá, Colombia
3 University of Prince Edward Island, 550 University Avenue, Charlottetown, PE C1A 4P3, Canada

Abstract

Introduction:

New strategies have been arisen to set a rapid and effective screening for selection of microorganism with bioactive potential. This study suggests that combination of physicochemical pretreatments and taxonomic dereplication of microbial collections through MALDI-TOF MS, facilitates the detection of low abundance actinobacteria with potential as a source of antimicrobial agents.

Material and Methods:

An unstudied microbial community from a tropical river sediment in Colombian Orinoquía is described, applying an extended cultivation strategy using physicochemical pretreatments, biological screenings and taxonomic dereplication through MALDI-TOF MS approach.

Results:

Actinobacteria-like isolates (790) were growth and their antimicrobial activity was assessed against methicillin-resistant Staphylococcus aureus, Vancomycin-resistant Enterococcus faecium, extended-spectrum β-lactamase Klebsiella pnumoniae, and clinical isolates of Cladosporium cladosporioides and Epicoccum nigrum. Seventy-eight isolates, belonging to the Streptomycetaceae family according to 16S rDNA analysis were found to have antimicrobial activity and were categorized as low abundance actinobacteria by MALDI-TOF MS.

Conclusion:

The results suggest that combination of physicochemical pretreatments and taxonomic dereplication of microbial collections through MALDI-TOF MS, facilitates the detection of low abundance actinobacteria with potential as a source of antimicrobial agents.

Keywords: Actinobacteria, Antimicrobial, Bioprospecting, MALDI-TOF MS, Physicochemical pretreatments, Taxonomic dereplication.


Article Information


Identifiers and Pagination:

Year: 2018
Volume: 12
First Page: 181
Last Page: 194
Publisher Id: TOMICROJ-12-181
DOI: 10.2174/1874285801812010181

Article History:

Received Date: 22/2/2018
Revision Received Date: 15/05/2018
Acceptance Date: 16/05/2018
Electronic publication date: 31/05/2018
Collection year: 2018

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© 2018 Arango et al.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: (https://creativecommons.org/licenses/by/4.0/legalcode). This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


* Address correspondence to this author at the Universidad de La Sabana Autop. Norte Km. 7, Campus del Común, Chia, Colombia, Ext 25208, Tel: +57 1 8615555; Fax: +57 1 8615555; Email: luisdb@unisabana.edu.co


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