RESEARCH ARTICLE


Whole Genome Sequencing of Klebsiella pneumoniae Strain Unravels a New Model for the Development of Extensive Drug Resistance in Enterobacteriaceae



Mubarak Alfaresi*
College of Medicine, University of Sharjah, Sharjah, UAE


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Creative Commons License
© 2018 Mubarak Alfaresi.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: (https://creativecommons.org/licenses/by/4.0/legalcode). This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

* Address correspondence to this author at the College of Medicine, University of Sharjah, Sharjah, UAE; E-mail: uaenow@eim.ae


Abstract

Introduction:

Increased incidence of carbapenem-resistant Enterobacteriaceae (CRE) has been reported worldwide. The WHO warns about the imminent risk to global health if the spread of resistant bacteria is not contained.

Materials and Methods:

Here, single molecule real time sequencing was used to analyse the whole genome and resistome of SKGH01, a strain of Klebsiella pneumoniae.

Results and Discussions:

The data showed that SKGH01 was resistant to all commercially available antibiotics. A complete account of extensively drug-resistant (XDR) CRE at a genomic level and the entire location map of all antibiotic resistance components are here presented. Additionally, this work proposes a model of XDR acquisition in Enterobacteriaceae.

Keywords: Klebsiella pneumoniae, Extensive drug resistance (XDR), Whole genome sequencing, Antibiotics, WHO, Enterobacteriaceae.