Effect of Solvents on Phytochemicals Content and Antioxidant Activity of Ganoderma lucidum
Moyen Uddin Pk1, 2, 3, Rabiul I. Talukder3, Mohammad K. I. Sarkar4, *, Tasnia Rahman4, Rumana Pervin5, Matiar Rahman5, Elina A. Zenat6, Lima Akther7
1 Institute of Biological Science, University of Rajshahi, Rajshahi, Bangladesh
2 Department of Biochemistry & Molecular Biology, University of Rajshahi, Rajshahi, Bangladesh
3 Department of Biochemistry, Primeasia University, Dhaka, Bangladesh
4 School of Environmental Science and Management, Independent University Bangladesh (IUB), Dhaka, Bangladesh
5 Department of Biochemistry & Molecular Biology, University of Rajshahi, Bangladesh
6 Bangladesh Council of Scientific and Industrial Research (BCSIR), Dhaka, Bangladesh
7 Department of Chemistry, Comilla University, Bangladesh
The aim of this study was to assess the induction of solvents on the total phenol and flavonoid content and also the antioxidant activity of Ganoderma lucidum extracts.
Materials & Methods:
In this study, two concentrations (100% and 75%) of diethyl ether, ethanol, butanol, chloroform, and acetone were used as extractants of Ganoderma lucidum. Total phenol and flavonoid contents were measured by spectrophotometric methods and 1, 1-diphenyl-2-picrylhydrazyl (DPPH). Free radical scavenging assay was used for the investigation of antioxidant activity.
Results & Discussion:
Extractants significantly affected the % yield of extract, the quantity of phenol and flavonoids and antioxidant activity of Ganoderma lucidum mushroom. The highest extraction yield, around 38%, was achieved by 75% acetone, followed by 100% acetone (about 36%) and 75% chloroform (approximate 21%). Hydro-acetone extract exhibited the most significant antioxidative properties (EC50 value; 645.55 µg/mL) comprised of a higher total of phenol content. In conclusion, the total phenol content encouraged the antioxidative potential of Ganoderma lucidum mushroom.
These findings indicate that the selective extraction of Ganoderma lucidum shows significant biological activities.
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* Address correspondence to this author at the School of Environmental Science and Management, Independent University Bangladesh (IUB), Dhaka, Bangladesh Tel: +8801711230892; E-mail: firstname.lastname@example.org