The Open Microbiology Journal

ISSN: 1874-2858 ― Volume 13, 2019

Effect of Solvents on Phytochemicals Content and Antioxidant Activity of Ganoderma lucidum

Moyen Uddin Pk1, 2, 3, Rabiul I. Talukder3, Mohammad K. I. Sarkar4, *, Tasnia Rahman4, Rumana Pervin5, Matiar Rahman5, Elina A. Zenat6, Lima Akther7
1 Institute of Biological Science, University of Rajshahi, Rajshahi, Bangladesh
2 Department of Biochemistry & Molecular Biology, University of Rajshahi, Rajshahi, Bangladesh
3 Department of Biochemistry, Primeasia University, Dhaka, Bangladesh
4 School of Environmental Science and Management, Independent University Bangladesh (IUB), Dhaka, Bangladesh
5 Department of Biochemistry & Molecular Biology, University of Rajshahi, Bangladesh
6 Bangladesh Council of Scientific and Industrial Research (BCSIR), Dhaka, Bangladesh
7 Department of Chemistry, Comilla University, Bangladesh



The aim of this study was to assess the induction of solvents on the total phenol and flavonoid content and also the antioxidant activity of Ganoderma lucidum extracts.

Materials & Methods:

In this study, two concentrations (100% and 75%) of diethyl ether, ethanol, butanol, chloroform, and acetone were used as extractants of Ganoderma lucidum. Total phenol and flavonoid contents were measured by spectrophotometric methods and 1, 1-diphenyl-2-picrylhydrazyl (DPPH). Free radical scavenging assay was used for the investigation of antioxidant activity.

Results & Discussion:

Extractants significantly affected the % yield of extract, the quantity of phenol and flavonoids and antioxidant activity of Ganoderma lucidum mushroom. The highest extraction yield, around 38%, was achieved by 75% acetone, followed by 100% acetone (about 36%) and 75% chloroform (approximate 21%). Hydro-acetone extract exhibited the most significant antioxidative properties (EC50 value; 645.55 µg/mL) comprised of a higher total of phenol content. In conclusion, the total phenol content encouraged the antioxidative potential of Ganoderma lucidum mushroom.


These findings indicate that the selective extraction of Ganoderma lucidum shows significant biological activities.

Keywords: : Edible mushroom, Free radical scavenging, Chronic diseases, Functional foods, Antioxidative potential, Ganoderma lucidum mushroom.

Article Information

Identifiers and Pagination:

Year: 2019
Volume: 13
First Page: 10
Last Page: 15
Publisher Id: TOMICROJ-13-10
DOI: 10.2174/1874285801913010010

Article History:

Received Date: 16/10/2018
Revision Received Date: 10/12/2018
Acceptance Date: 18/12/2018
Electronic publication date: 31/01/2019
Collection year: 2019

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© 2019 Uddin Pk et al.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: ( This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

* Address correspondence to this author at the School of Environmental Science and Management, Independent University Bangladesh (IUB), Dhaka, Bangladesh Tel: +8801711230892; E-mail:


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