RESEARCH ARTICLE


Effect of Solvents on Phytochemicals Content and Antioxidant Activity of Ganoderma lucidum



Moyen Uddin Pk1, 2, 3, Rabiul I. Talukder3, Mohammad K. I. Sarkar4, *, Tasnia Rahman4, Rumana Pervin5, Matiar Rahman5, Elina A. Zenat6, Lima Akther7
1 Institute of Biological Science, University of Rajshahi, Rajshahi, Bangladesh
2 Department of Biochemistry & Molecular Biology, University of Rajshahi, Rajshahi, Bangladesh
3 Department of Biochemistry, Primeasia University, Dhaka, Bangladesh
4 School of Environmental Science and Management, Independent University Bangladesh (IUB), Dhaka, Bangladesh
5 Department of Biochemistry & Molecular Biology, University of Rajshahi, Bangladesh
6 Bangladesh Council of Scientific and Industrial Research (BCSIR), Dhaka, Bangladesh
7 Department of Chemistry, Comilla University, Bangladesh


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Creative Commons License
© 2019 Uddin Pk et al.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: (https://creativecommons.org/licenses/by/4.0/legalcode). This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

* Address correspondence to this author at the School of Environmental Science and Management, Independent University Bangladesh (IUB), Dhaka, Bangladesh Tel: +8801711230892; E-mail: kisarkar1981@gmail.com


Abstract

Introduction:

The aim of this study was to assess the induction of solvents on the total phenol and flavonoid content and also the antioxidant activity of Ganoderma lucidum extracts.

Materials & Methods:

In this study, two concentrations (100% and 75%) of diethyl ether, ethanol, butanol, chloroform, and acetone were used as extractants of Ganoderma lucidum. Total phenol and flavonoid contents were measured by spectrophotometric methods and 1, 1-diphenyl-2-picrylhydrazyl (DPPH). Free radical scavenging assay was used for the investigation of antioxidant activity.

Results & Discussion:

Extractants significantly affected the % yield of extract, the quantity of phenol and flavonoids and antioxidant activity of Ganoderma lucidum mushroom. The highest extraction yield, around 38%, was achieved by 75% acetone, followed by 100% acetone (about 36%) and 75% chloroform (approximate 21%). Hydro-acetone extract exhibited the most significant antioxidative properties (EC50 value; 645.55 µg/mL) comprised of a higher total of phenol content. In conclusion, the total phenol content encouraged the antioxidative potential of Ganoderma lucidum mushroom.

Conclusion:

These findings indicate that the selective extraction of Ganoderma lucidum shows significant biological activities.

Keywords: : Edible mushroom, Free radical scavenging, Chronic diseases, Functional foods, Antioxidative potential, Ganoderma lucidum mushroom.