Antibiotic Resistance Pattern and Frequency of PER-1, SHV-1 and AMPC Type B-Lactamase Genes in Pseudomonas aeruginosa Isolated from Clinical Samples
Fatemeh F. Amoudizaj1, *, Elnaz Aghayi2, Milad G. Matin3, Nayemeh Soltani3, Pejman Mala3
1 Department of Genetic, Tabriz Branch, Islamic Azad University, Tabriz, Iran
2 Department of Molecular Biology, Bonab Branch, Islamic Azad University, Bonab, Iran
3 Department of Microbiology, Urmia Branch, Islamic Azad University, Urmia, Iran
The existence of Extended Spectrum B-lactamase (ESBL) genes plays an important role in spreading B-lactam antibiotic resistance in the producing strains of these enzymes. The resistance of gram-negative bacteria, such as Pseudomonas aeruginosa, to different antimicrobial agents, especially B-lactams, has increasingly been reported.
This study was conducted to determine the prevalence of TEM-1and VEB-1 beta-lactamases gene in P. aeruginosa isolates through Polymerase Chain Reaction (PCR) method.
100 clinical isolates of P. aeruginosa were collected from different clinical samples. The antibiotic susceptibility was examined by the disc diffusion method. The presence of PER-1, SHV-1 and AMPC genes was detected by PCR method.
Out of the studied P. aeruginosa isolates, 7, 9 and 37 isolates were positive for PER-1, SHV-1 and AMPC B-lactamases resistance genes, respectively. Patients with urinary infection had the most resistant isolates. All isolates (100%) were sensitive to polymyxin B.
Antibiotic resistance in isolates of Pseudomonas can be caused by B-lactamases resistance genes. Noticing the increasing rate of the ESBLs producing strains, using the appropriate treatment protocol based on the antibiogram pattern of the strains is highly recommended.
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* Address correspondence to this author at Department of Genetic, Tabriz Branch, Islamic Azad University, Tabriz, Iran; E-mail: fatemeh.firoozi96@
yahoo.com; Tel: +98-9395572387