The Open Microbiology Journal




ISSN: 1874-2858 ― Volume 13, 2019
RESEARCH ARTICLE

Detection of OXA Beta Lactamases Among Clinical Isolates of Acinetobacter baumannii Isolated from Tehran Hospitals, Iran



Reza Ranjbar1, *, Shahin Zayeri1, Davoud Afshar2, Shohreh Farshad3
1 Molecular Biology Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran
2 Department of Microbiology and Virology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran
3 Alborzi Clinical Microbiology Research Center, Namazi Hospital, Shiraz University of Medical Sciences, Shiraz, IR Iran

Abstract

Background and Objective:

Acinetobacter baumannii is a non-motile Gram-negative bacterial pathogen with the history of vast resistant to antibiotics. The aim of this study was to determine the possibility of existence of OXAs genes among clinical isolates of A. baumannii obtained from Tehran hospitals.

Materials and Methods:

A total of 101 isolates were identified as A. baumannii by common biochemical and molecular tests. The susceptibility to different antibiotics was assessed with Kirby-Bauer disk diffusion method. Phenotypic Detection of MBLs was performed with CDT test and PCR assay was also performed for detection of blaOXA-23-like, blaOXA-24-like, blaOXA-40-like,blaOXA-51-like,blaOXA-58-like and blaOXA-143-like genes

Results:

All isolates of A. baumannii showed high-level of resistance to all antibiotics except for Polymyxin B. The blaOXA-51 like genes was found in all of the isolates and the prevalence of blaOXA-143like, blaOXA-23like,blaOXA-40like and blaOXA-24like were 56%, 45.45%, 33% and 11.8%, respectively.

Conclusion:

The blaOXA-51-like was the predominant mechanism of resistance to imipenem in A. baumannii and therefore, early recognition of carbapenem-resistant A. baumannii isolates is a useful tools to prevent their spreading within the hospital environment.

Keywords: Acinetobacter baumannii, Carbapenemase, PCR, Gram-negative, Phenotypic detection, Polymyxin B.


Article Information


Identifiers and Pagination:

Year: 2019
Volume: 13
First Page: 68
Last Page: 72
Publisher Id: TOMICROJ-13-68
DOI: 10.2174/1874285801913010068

Article History:

Received Date: 04/11/2018
Revision Received Date: 28/02/2019
Acceptance Date: 10/3/2019
Electronic publication date: 26/03/2019
Collection year: 2019

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© 2019 Ranjbar et al.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: (https://creativecommons.org/licenses/by/4.0/legalcode). This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


* Address correspondence to this author at the Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran; Tel: +98-21-88039883; Fax: +98-21-88039883; E-mail: ranjbarre@gmail.com


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