1 Molecular Biology Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran
2 Department of Microbiology and Virology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran
3 Alborzi Clinical Microbiology Research Center, Namazi Hospital, Shiraz University of Medical Sciences, Shiraz, IR Iran
Background and Objective:
Acinetobacter baumannii is a non-motile Gram-negative bacterial pathogen with the history of vast resistant to antibiotics. The aim of this study was to determine the possibility of existence of OXAs genes among clinical isolates of A. baumannii obtained from Tehran hospitals.
Materials and Methods:
A total of 101 isolates were identified as A. baumannii by common biochemical and molecular tests. The susceptibility to different antibiotics was assessed with Kirby-Bauer disk diffusion method. Phenotypic Detection of MBLs was performed with CDT test and PCR assay was also performed for detection of blaOXA-23-like, blaOXA-24-like, blaOXA-40-like,blaOXA-51-like,blaOXA-58-like and blaOXA-143-like genes
All isolates of A. baumannii showed high-level of resistance to all antibiotics except for Polymyxin B. The blaOXA-51 like genes was found in all of the isolates and the prevalence of blaOXA-143like, blaOXA-23like,blaOXA-40like and blaOXA-24like were 56%, 45.45%, 33% and 11.8%, respectively.
The blaOXA-51-like was the predominant mechanism of resistance to imipenem in A. baumannii and therefore, early recognition of carbapenem-resistant A. baumannii isolates is a useful tools to prevent their spreading within the hospital environment.
Keywords: Acinetobacter baumannii, Carbapenemase, PCR, Gram-negative, Phenotypic detection, Polymyxin B.
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