RESEARCH ARTICLE


Statistical Optimization by Response Surface Methodology to Enhance Lipase Production by Aspergillus fumigatus



Akshita Mehta, Roji Sharma, Reena Gupta*
Department of Biotechnology, Himachal Pradesh University, Summerhill, Shimla-171 005, India


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Creative Commons License
© 2019 Mehta et al.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: (https://creativecommons.org/licenses/by/4.0/legalcode). This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

* Address correspondence to this author at the Department of Biotechnology, Himachal Pradesh University, Summerhill, Shimla 171 005, India;
Tel: 911772831948; Fax: 911772831948; E-mail: reenagupta_2001@yahoo.com


Abstract

Background:

Lipases have various commercial applications and microorganisms serve as a potential source of production.

Objective:

The aim of this paper was to study the effect of interactions among different production parameters on lipase yield of Aspergillus fumigatus.

Method:

Plackett Burman and Central Composite Design (CCD) were established by using Design Expert software 10.0.

Results:

In the present study, interactions were studied for six different variables such as inoculum size, pH, temperature, galactose concentration, peptone concentration and incubation time. In Plackett-Burman design, galactose concentration, peptone concentration, pH and incubation time were found to be important factors. Using the statistical approach, the optimum factors were found to be as: galactose concentration (1.5%), peptone concentration (1.8%), pH (10.0) and incubation time (72 h) at 45°C under response surface curves. Upon statistical analysis, the coefficient of determination (R2) obtained was 0.9318 which showed that the model was significant.

Conclusion:

The statistical tools used predicted the optimal conditions for the production of the lipase. The optimized parameters were galactose concentration 1.5%, peptone concentration 1.4%, temperature 45°C, pH 10.0 and incubation time of 72 h for obtaining a maximum lipase activity of 6.22 U/ml.

Keywords: Lipase, Response Surface Methodology, Plackett-Burman, Production, Coefficient of Determination, Statistical analysis.