RESEARCH ARTICLE
Antibacterial Activity of Honey against Methicillin-Resistant and Sensitive Staphylococcus Aureus Isolated from Patients with Diabetic Foot Ulcer
Arezou Mirzaei1, †, Kolsoom Shirzadi Karamolah2, †, Mina Pourmbarak Mahnaie3, Fatemeh Mousavi2, Parisa Mehrabi Moghadam1, Hassan Mahmoudi4, *
Article Information
Identifiers and Pagination:
Year: 2020Volume: 14
First Page: 260
Last Page: 265
Publisher ID: TOMICROJ-14-260
DOI: 10.2174/1874434602014010260
Article History:
Received Date: 28/4/2020Revision Received Date: 25/8/2020
Acceptance Date: 4/9/2020
Electronic publication date: 25/11/2020
Collection year: 2020
open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: (https://creativecommons.org/licenses/by/4.0/legalcode). This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
Introduction:
Staphylococcus aureus is the most important causative agent of wound infections, including diabetic foot ulcers. Honey is a very useful nutrient with antimicrobial properties and other biological properties such as antitumor, anti-inflammatory, antioxidant and antiviral properties. The aim was to examine the antibacterial activity of honey against methicillin-resistant and sensitive S. aureus (MRSA and MSSA) isolated from patients with diabetic foot ulcers.
Methods:
This cross-sectional study was performed from January 2019 to December 2019. Twenty S. aureus isolates were collected from patients with diabetic foot ulcers. Different concentrations (100%, 70%, 50%, 25% vol/vol) of honey were studied. Dilutions of honey solutions were examined to determine the minimum inhibitory concentration (MIC) against S. aureus. MICs were determined by spectrophotometric assay at 620 nm.
Results:
All strains showed sensitivity to honey with MIC equal to 25% (vol/vol). The MIC (%) values of honey for all studied S. aureus (MRSA and MSSA) isolates ranged between 18-100% (v/v).
Conclusion:
Honey with confirmed, antibacterial activity has the potential to be an efficient treatment complementary for diabetic foot ulcers infected or at risk of infection with S. aureus.