Cloning, Purification, and Partial Characterization of the Halobacterium sp. NRC-1 Minichromosome Maintenance (MCM) Helicase
Nozomi Sakakibara*, 1, Mimi Han1, Claire R Rollor1, Rebecca C Gilson1, Courtney Busch2, Gunyoung Heo1, Zvi Kelman1
1 University of Maryland Biotechnology Institute, Center for Advanced Research in Biotechnology, 9600 Gudelsky Drive, Rockville, MD 20850, USA
2 Department of Cell Biology and Molecular Genetics, University of Maryland College Park, College Park MD 20742, USA
The MCM gene from the archaeon Halobacterium, with and without its intein, was cloned into an Escherichia coli expression vector, overexpressed and the protein was purified and antibodies were generated. The antibodies were used to demonstrate that in vivo only the processed enzyme, without the intein, could be detected.
Received Date: 29/11/2007 Revision Received Date: 12/2/2008 Acceptance Date: 12/2/2008 Electronic publication date: 12/3/2008 Collection year: 2008
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2008 Bentham Science Publishers Ltd.
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* Address correspondence to this author at the University of Maryland Biotechnology Institute, Center for Advanced Research in Biotechnology, 9600 Gudelsky Drive, Rockville, MD 20850, USA; E-mail: firstname.lastname@example.org