A Comparative Study of the Outer Membrane Proteome from an Atypical and a Typical Enteropathogenic Escherichia coli
Carla R Taddei1, 4, 5, *, Fernanda F Oliveira1, 4, Roxane M. F Piazza1, Adriana F. Paes Leme2, Clécio F Klitzke2, Solange M. T Serrano2, Marina B Martinez4, Waldir P Elias1, Osvaldo A Sant´Anna3
1 Laboratório de Bacteriologia, Av. Vital Brasil, 1500 - 05503-900, Instituto Butantan, São Paulo, Brazil
2 Laboratório Especial de Toxinologia Aplicada, Av. Vital Brasil, 1500 - 05503-900, Instituto Butantan, São Paulo, Brazil
3 Laboratório de Imunoquímica, Av. Vital Brasil, 1500 - 05503-900, Instituto Butantan, São Paulo, Brazil
4 Laboratório de Microbiologia Clínica, Departamento de Análises Clínicas, Faculdade de Ciências Farmacêuticas
5 Escola de Artes, Ciências e Humanidades, Av. Prof. Lineu Prestes, 580 – 05508-900, Universidade de São Paulo, Brazil
This study compared the proteomic profile of outer membrane proteins (OMPs) from one strain of atypical enteropathogenic Escherichia coli (aEPEC) and one of typical EPEC (tEPEC). The OMPs fractions were obtained using sarcosine extraction, and analyzed by one- and two-dimensional gel electrophoresis (1DE and 2DE, respectively). The 1DE OMPs analysis of typical and atypical EPEC evidenced similar patterns; however, the 2DE OMP profile from the aEPEC revealed more protein spots in the 40- to 70-kDa region. 2DE image analysis identified 159 protein spots in both strains whereas 53 protein spots were observed only in tEPEC and 128 were observed only in aEPEC. Remarkably, 41.5% of aEPEC spots showed higher levels of expression compared to tEPEC, some of which with two, others four or even five times more. Twenty-four selected spots were identified using MALDI-TOF mass spectrometry and they corresponded to proteins involved in cell structure and metabolism, as well as in gene regulation. Some of these proteins showed similarity with proteins identified in other E. coli pathotypes. Besides, the differential expression of some proteins in aEPEC may suggest that it could be related to their features that ascertain the adaptation to distinct environments and the worldwide spread distribution of these pathogens.
Keywords: Atypical and typical enteropathogenic Escherichia coli, proteome, OMP profiles.
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* Address correspondence to this author at the Laboratório de Microbiologia Clínica, Departamento de Análises Clínicas e Toxicológicas, Faculdade de Ciências Farmacêuticas. Prof. Lineu Prestes, 580 – bloco 17. Cep:05508900, Universidade de São Paulo, SP – Brazil; Tel: 55 11 30913636; Fax: 55 11 38132197; E-mail: email@example.com