Assessment of the Antimicrobial Activity of Few Saudi Arabian Snake Venoms
Abdulrahman K. Al-Asmari1, *, Rajamohamed Abbasmanthiri1, Nasreddien M. Abdo Osman1, Yunus Siddiqui1, Faisal Ahmed Al-Bannah2, Abdulgadir M. Al-Rawi3, Sarah A. Al-Asmari4
1 Department of Research Center
3 Family & Community Medicine
4 Dentistry, Prince Sultan Military Medical City, Riyadh, Saudi Arabia
Venoms of two cobras, four vipers, a standard antibiotic and an antimycotic, were evaluated comparatively, as antimicrobials.
Six venom concentrations and three of the standard antibiotic and the antimycotic were run in micro-dilution and diffusion plates against the microorganisms.
Echis pyramidum, Echis coloratus
and Cerastes cerastes gasperettii highest venom concentrations gave significant growth inhibition zones (GIZ) with
respect to a negative control, except Bitis arietans, whose concentrations were significant. The cobra Walterinnesia aegyptia
had significant venom concentrations more than Naja haje arabica. The Staphylococcus aureus Methicillin Resistant
(MRSA) bacterium was the most susceptible, with a highly (P < 0.001) significant GIZ mean difference followed by
the Gram positive Staphylococcus aureus, (P < 0.001), Escherichia coli (P < 0.001), Enterococcus faecalis (P < 0.001)
and Pseudomonas aeruginosa which, had the least significance (P < 0.05). The fungus Candida albicans was resistant to
both viper and cobra venoms (P > 0.05). The antibiotic Vancomycin was more effective than snake venoms though, they
were more efficient in inhibiting growth of the resistant Pseudomonas aeruginosa. This antibiotic was also inactive
against the fungus, whilst its specific antifungal Fungizone was highly efficient with no antibacterial activity.
These findings showed that snake venoms had antibacterial activity comparable to antibiotics, with a directly proportional
relationship of venom concentration and GIZ, though, they were more efficient in combatting resistant types of
bacteria. Both venoms and the standard antibiotic, showed no antifungal benefits.
Keywords: Agar diffusion, antimicrobial, Echis pyramidum, fungizone, GIZ, venoms..
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* Address correspondence to this author at the Research Center, Prince Sultan
Military Medical City, PO Box 7897 (777-S), Riyadh 11159, Saudi
Arabia; Tel: +966 (1) 4777714; Ext: 25100; Fax: +966 (1) 4786601;