The quantitative analysis of blood vessel volumes from magnetic resonance angiograms (MRA) or μCT images
is difficult and time-consuming. This fact, when combined with a study that involves multiple scans of multiple subjects,
can represent a significant portion of research time. In order to enhance analysis options and to provide an automated and
fast analysis method, we developed a software plugin for the ImageJ and Fiji image processing frameworks that enables
the quick and reproducible volume quantification of blood vessel segments. The novel plugin named Volume Calculator
(VolCal), accepts any binary (thresholded) image and produces a three-dimensional schematic representation of the
vasculature that can be directly manipulated by the investigator.
Using MRAs of the mouse hindlimb ischemia model, we demonstrate quick and reproducible blood vessel volume
calculations with 95 – 98% accuracy. In clinical settings this software may enhance image interpretation and the speed of
data analysis and thus enhance intervention decisions for example in peripheral vascular disease or aneurysms.
In summary, we provide a novel, fast and interactive quantification of blood vessel volumes for single blood vessels or
sets of vessel segments with particular focus on collateral formation after an ischemic insult.