Two different approaches to the creation of “rigid” (gel-like) DNA particles (or DNA nanoconstructions) are
considered. The physicochemical approach is based on the formation of nanobridges between double-stranded DNA
molecules ordered in quasinematic layers of cholesteric liquid-crystalline dispersion (CLCD) particles or their salting-out.
This approach represents by itself a “chemical gelation” of DNA molecules realized inside the nanometric DNA particles.
The formed “rigid” DNA particles have unique physicochemical properties. The nanotechnological approach is based on
induction of “physical gelation” as a result of the formation of Au-clusters in the “free” space between double-stranded
DNA molecules ordered in CLCD particles. This approach results in the formation of a new “rigid” DNA material. Its distinctive
peculiarity, in contrast to the chemically bonded “rigid” DNA particles, consists in a very weak binding affinity to
the nuclear membrane filter used for atomic force microscopy. This opens up a possibility for easy manipulations with
CLCD particles doped with gold nanoparticles.