Long-Term Monitoring of Post-Stroke Plasticity After Transient Cerebral Ischemia in Mice Using In Vivo and Ex Vivo Diffusion Tensor MRI
C Granziera*, 1, 2, H D’Arceuil*, #, 1, L Zai3, P.J Magistretti4, A.G Sorensen1, A.J de Crespigny1
1 Athinoula A. Martinos Center for Biomedical Imaging, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA, USA
2 Department of Neurology, CHUV, Lausanne, Switzerland
3 Laboratories for Neuroscience Research in Neurosurgery, Children’s Hospital, Harvard Medical School, Charlestown, MA, USA
4 Brain Mind Institute, Ecole Polytechnique Federale de Lausanne (EPFL) and Centre de Neurosciences Psychiatriques, Departement de Psychiatrie, CHUV, Lausanne, Switzerland
We used a murine model of transient focal cerebral ischemia to study: 1) in vivo DTI long-term temporal evolution of the apparent diffusion coefficient (ADC) and diffusion fractional anisotropy (FA) at days 4, 10, 15 and 21 after stroke 2) ex vivo distribution of a plasticity-related protein (GAP-43) and its relationship with the ex vivo DTI characteristics of the striato-thalamic pathway (21 days).
All animals recovered motor function. In vivo ADC within the infarct was significantly increased after stroke. In the stroke group, GAP-43 expression and FA values were significantly higher in the ipsilateral (IL) striatum and contralateral (CL) hippocampus compared to the shams. DTI tractography showed fiber trajectories connecting the CL striatum to the stroke region, where increased GAP43 and FA were observed and fiber tracts from the CL striatum terminating in the IL hippocampus.
Our data demonstrate that DTI changes parallel histological remodeling and recovery of function.
Received Date: 13/8/2007 Revision Received Date: 11/10/2007 Acceptance Date: 18/10/2007 Electronic publication date: 13/11/2007 Collection year: 2007
Full-Text HTML Views: 1090 Abstract HTML Views: 667 PDF Downloads: 228 Total Views/Downloads: 1985
Full-Text HTML Views: 646 Abstract HTML Views: 466 PDF Downloads: 150 Total Views/Downloads: 1262 Geographical View
2007 Bentham Science Publishers Ltd
* Address correspondence to this author at the Athinoula A. Martinos Center for Biomedical Imaging, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA, USA; E-mail: email@example.com# Both authors contributed equally to this mauscript.