Centella asiatica L. or locally known as Pegaga nyonya is a weekly aromatic smelling herb that belongs to a genus of the plant family Apiaceae (Umbelliferare). It has been used widely in folk medicine for hundreds of years to treat a wide range of illness. In this study, the mutagenic potential of C. asiatica aerial parts (leaves and stems) and roots in aqueous extracts were determined using the Ames test. The method involved was pre-incubation on Salmonella typhimurium TA 98 and TA 100 bacterial strains in the presence and absence of metabolic activator S9 system. The extracts were evaluated using two-fold value of the number of revertant’s colony in negative control plate as cut-off point, to determine the mutagenicity effects. The results showed that all aqueous extracts of the aerial parts and roots of C.asiatica were non mutagenic because it shown no significance difference (p<0.05) when compared to negative control towards TA98 and TA100 strain with and without S9 metabolic activation for all concentration studied. In conclusion, C. asiatica aerial parts and roots aqueous extract were non mutagenic on both S.typhimurium strains and can be used as part of traditional medicine.