Thrombosis and cardiovascular-related diseases can be caused by fibrin aggregation. Thrombolytic agents have
been used to treat thrombolytic problems and cardiovascular-related diseases. There had been searches for new
thrombolytic agents from bacteria, fungi, insects and fermented foods. To name a few, recently fungi such as Aspergillus
oryzae KSK-3, Fusarium sp. CPCC 480097 and Fusarium sp. BLB were found able in producing protease fibrinolytic
enzymes. Potential protease fibrinolytic enzymes were observed from Lignosus rhinocerus bought from Orang Asli of
Sungei Perak. Out of the six (6) sclerotia used, one of them, LR-1, produced the biggest clear zone on Skim Milk Agar
with a diameter of 1.31 cm. Fibrin plate assay of LR-1 showed that it produced a clear zone of 0.97 cm when the plate was
incubated for 18 hours at 37°C Leaves of Hibiscus sp. were sterilised with 70% alcohol and 70% sodium hypochlorite.
The leaves were then cut into pieces of 4 cm and placed on Potato Dextrose Agar plate. Fungi grown from the leave
pieces were sub cultured onto new Potato Dextrose Agar. This then showed that Lignosus rhinocerus had protease and
fibrinolytic activities. Sixteen (16) endophytic fungi were isolated from Hibiscus sp. leaves from six locations in Shah
Alam, Selangor. Two endophytic fungi, FH-3 and S-13 showed protease activities; clear zones of 2.89 cm and 2.42 were
seen.after 4 days incubation at 27°C. FH-3 had fibrinolytic activity which was observed by the 1.98 cm diameter clear
zone on fibrin plate.