The Open Conference Proceedings Journal
(Biological Sciences, Chemical Sciences, Physical Sciences, Medicine, Engineering & Technology)
(Discontinued)
ISSN: 2210-2892 ― Volume 10, 2020
Optimization of Extraction Method and Qualitative FT-NMR Analysis on Andrographis Paniculata Leaves
The Open Conference Proceedings Journal, 2013, 4: 133
Azizul Isha , Mohamad Allif Suyut , Nur A’thifa Yusof , Intan Safinar Ismail
Laboratory of Natural Products, Institute of Bioscience, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor Darul Ehsan, Malaysia.
Electronic publication date 01/3/2013
[DOI: 10.2174/22102892013040101333 ]
Abstract:
A study was conducted to optimize the extraction conditions of Andrographis paniculata leaves. Four different solvents, methanol (MeOH), dichloromethane (DCM), ethyl acetate (EtOAc) and water (H2O) were used in a period of 1, 3, 5 or 7 hours at set water-bath temperature of 25, 30, 40 or 60°C. The extraction dried mass yield of about 200 ± 0.1 mg fresh cut leaves was measured, and 1D-Nuclear Magnetic Resonance (NMR) profiles were employed to correlate the crude extract weight to the percentage of the main compound, andrographolide, present. Methanol was shown to be the best solvent with 13.75% yield in 3 hours extraction at 40°C. The NMR peak intensity analysis of the major compound (andrographolide) also in support of these obtained parameters.