Calliandra is a genus of flowering plant that is widely distributed in the tropical and subtropical regions of southern Asia,
Africa, Australia and America. Calliandra surinamensis is a large multiple trunked, low branching evergreen shrub that has
silky leaflets, which are glossy copper when new, turning to a dark metallic green. The flower is pink colored with pleasant
fragrant. Calliandra surinamensis or its local name Pink Powderpuff (Kaliandra bunga merah) is belongs to the family of
Leguminosae and subfamily of Mimosoideae. The flower pigment of Calliandra surinamensis is used folklorically as a
colorant and dye. Besides, in folk medicine, the stem bark of this plant was reported to be able to treat various diseases and
infections such as cough, wound healing, inflammation and antimicrobial activities . The antioxidant or free radical
scavenging activities of the stem bark using standard experimental procedures has been reported . The flower of Calliandra surinamensis were extracted by successive extraction. About 70 grams of the Calliandra surinamensis flower was defatted in
methanol (40°C – 60°C) for 48 hours and repeated for three times to yield 40 grams of methanol extract. The phytochemical
screening on the crude extract was tested for flavonoid and alkaloids. The phytochemical procedures adopted were vacuum
liquid chromatography (VLC), column chromatography, radial chromatography and preparative thin layer chromatography.
Phytochemical investigation of Calliandra surinamensis flower has afforded three compounds which are 3-orhamnosylquercitrin
(1), 5’-prenylated kaempferol (2) and quercetin (3). The structural elucidation of isolated compounds were
accomplished by spectroscopic method such as 1D-NMR (1H, 13C, DEPT) and 2D-NMR (COSY, HMQC, HMBC), UV, IR,
MS and comparison with published data.