A metabolite profiling approach based on 1H NMR was used to investigate time-dependent metabolic changes during
Ganoderma boninense (Gb) infection in oil palm leaf samples. Leaf samples taken in every month starting from 3 month old
seedlings were flashed frozen in liquid nitrogen at sampling site and were later subjected to grinding, extraction in CD3OD +
0.05%(v/v) TMS and filtration procedures to obtain less polar metabolites. Using biostatistical analysis, differences between
non-Gb inoculated and Gb inoculated samples were found to be insignificant, similarly with the phenotypic observation for
both. However, disease progression analysis indicated the up-regulation of amino acid compounds such as 2-aminobutyrate,
carnitine, glutamate and O-acetylcarnitine and down regulation of sugar and some organic acid compounds throughout the 5
months post infection (mpi). Further understanding of each compound related to plant disease signaling is under investigation.