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Rafflesia cantleyi is a parasitic flowering plant which belongs to Rafflesiaceae family. The parasitic plant grows on Tetrastigma
tuberculatum, a member of Vitaceae family. Also known as obligate holoparasite, Rafflesia cantleyi can be found in penisular
Malaysia as well as Borneo but in rare and limited distributions1. In this study, the alkaloid compounds in the flower bud of
Rafflesia cantleyi and the roots of its host, Tetrastigma tuberculatum are identified using phytochemical screening method and
HPLC technique. The sample of Rafflesia cantleyi and Tetrastigma tuberculatum were collected from Belum Valley, Banding
Lake, Perak. Air dried powdered samples were first extracted using dichloromethane followed by methanol. The methanol
extract of R.cantleyi and T.tuberculatum were then subjected to alkaloid screening test2. The results showed strong positive test
for T.tuberculatum indicating the presence of alkaloid compounds in T.tuberculatum. However, the screening test for
R.cantleyi showed negative result for alkaloid content. Both extract were then subjected to HPLC for alkaloid identification
using caffeine as standard. Based on the HPLC spectrum obtained, caffeine is presence in both R.cantleyi and T.tuberculatum.
The methanol extract of both samples were also tested for antioxidant activity by using DPPH free radical scavenging activity3.
Result from the DPPH method showed that the radical scavenging activity of R.cantleyi is 96.0% whereas for T.tuberculatum is
97.0% which is quiet high when compared to standard alpha tocopherol (natural antioxidant) and BHT (synthetic antioxidant)
which have the value of 97.0% and 96.0% antioxidant activities respectively.