It is strictly evident that honey contains antioxidant activity; however, constituents of honey responsible for its antioxidant
activity are still needed to be investigated. The aim of this study was to purify protein fraction on the basis of antioxidant
activity. Fourteen natural honey samples from North Africa and South Asia were evaluated for their antioxidant activities by
employing 1,1-Diphenyl-2-picrylhydrazyl (DPPH) radical assay. The scavenging activity of all honey samples ranged 18.7-
32.6%. A 62 kDa protein was isolated from honey by gel filtration chromatography followed by reverse phase HPLC. This
protein showed significant radical scavenging activity (54.6 %). This indicated the possible role of this protein in the
antioxidant and therapeutic properties of the natural honey.