Abstract:

In Malaysia, Alstonia spathulata Bl. is locally known as ‘pulai basong’ which distribute in peat forest. The separation of the chemical components from hexane crude extract (bark) of Alstonia spathulata was carried out using different chromatographic techniques (column chromatography and thin layer chromatography). Two compounds were isolated and identified as betaamyrin 1a and beta-amyrin acetate 1b. The compounds are reported for the first time from this plant. The structures of these compounds were elucidated based on the basis of detailed 1D-NMR (¹H, ¹³C and DEPT) and 2D-NMR (COSY, HSQC, HMBC) spectroscopic analysis, involving also comparison with data from the literature.