Pandanus amaryllifolius (PA), from Pandanaceae family, is widely distributed in Asia. This study determines
the interaction of the ethanolic extract of PA with cholinergic and histaminergic receptors in the guinea pig ileum.
The effect of PA was investigated in the isolated guinea pig ileum, suspended in organ baths containing aerated
Krebs solution at 36.9°C. Different doses of agonists (acetylcholine and histamine) were injected into the organ baths in the
presence of different doses of PA or selected antagonists (atropine or mepyramine). The contractions of the tissues were
recorded using PowerlabTM.
Results & Discussion:
Mepyramine and atropine showed competitive reversible antagonistic characteristics on the actions of
histamine or acetylcholine. Dose response curves of histamine and acetylcholine in the presence of different doses of PA
(1.0mg/mL, 2.5mg/mL and 5.0mg/mL) were shifted to right with significantly increased in the EC50 (p < 0.05). Through Schild
plot, the pA2 value obtained for PA on histaminergic receptors was 1.12 while equilibrium coefficient, KB was 1.31 x 10-1M.
On the other hand, the pA2 value for PA on cholinergic receptors acquired was 0.852 whereas KB was 7.12M.
PA extract has antimuscarinic and antihistaminic action in the guinea pig ileum. Further studies should be
conducted to determine the role of other types of receptors that could be involved in the action of PA on ileum.