Our previous study demonstrated the potent in vitro collagen biosynthesis stimulating and antioxidant activities of Volvariella volvacea extract. This study was performed the loading of V. volvacea extract (VV) in niosomes (NV). The physicochemical characterization of the VV loaded niosomes and gel containing niosome of the extract (GNV) was studied. Rat skin transdermal absorption by Franz diffusion cells at 6 hours of NV, GNV was compared with V. Volvacea extract solution (SV). GNV and NV showed higher chemical stability of total phenolic contents than SV. NV exhibited negative zeta potential values with the mean size of 254±20.32 nm. Both GNV and NV retarded the cumulative amounts and fluxes of the total phenolic in the extract in the first hour of skin permeation, while enhanced the skin permeation at the 6th hour in the experiment. GNV gave the highest percentages of the total phenolic content through rat skin to the receiving solution followed by NV and SV, respectively. This study has demonstrated the potential of gel containing niosomes of the V. volvacea extract appeared to be the suitable system for topical anti-aging application of V. volvacea extract because of the enhancement of chemical stability and rat skin transdermal absorption of V. volvacea extract.