3 Environmental and Life Sciences, Trent University, 1600 West Bank Drive, Peterborough, Ontario, K9J 7B8, Canada
A simple and fast high performance liquid chromatography-electrospray tandem mass spectrometry (LC-ESI-MS/MS) method has been developed for the analysis of brassinosteroids (BRs) in plants without derivatization.
The BRs (including castasterone, 24-epicastasterone, brassinolide and 24-epibrassinolide) have been extracted with ice cold 80% aqueous methanol solution.
Five different purification strategies have been tested for the purification and enrichment of BRs.
This analytical method was sensitive, reliable, rapid and applicable to trace analysis in complex plant samples.
open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: https://creativecommons.org/licenses/by/4.0/legalcode. This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
* Address correspondence to this author at the Department of Biology, Trent University, Peterborough, ON, K9J 7B8, Canada, Tel: 705-748-1011 7601, Fax: 705-748-1003; E-mail: email@example.com