Biomarkers of Fibroproliferative Healing in Fibrosing Idiopathic Interstitial Pneumonias
Martina Vasakova*, 1, Martina Sterclova 1, Eliska Stranska 2, Petra Mandakova 2, Jelena Skibova 3, Radoslav Matej 4
1 Department of Respiratory Medicine, Thomayer Hospital, Videnska 800, 14059 Prague 4, Czech Republic
2 Department of Pathology and Molecular Medicine, Charles University in Prague, 2nd Medical Faculty, V Uvalu 84, 15006 Prague 5, Czech Republic
3 Medical Statistics Unit, Institute for Clinical and Experimental Medicine, Videnska 1958/9, 14021 Prague 4, Czech Republic
4 Department of Pathology and Molecular Medicine, Thomayer Hospital, Videnska 800, 14059 Prague 4, Czech Republic
The main feature of fibrosing idiopathic interstitial pneumonias (fIIPs) is the fibroproliferative potential of underlying pathogenetic process. We hypothesize that the concentration of potential markers of fibroproliferative healing, PAR-2, TGF-β1, TNF-α and IL-4Rα in bronchoalveolar lavage fluid (BALF) differ in patients with fIIPs compared to controls (C).
Patients and Methods:
10 patients with fIIPs and 9 controls (C) were included to the study.
Concentrations of CD124 (IL4Rα), PAR-2, TGF-β1 and TNF-α in BALF were determined using the ELISA method.
We observed higher concentrations of IL4Rα (fIIPS 1499.4 pg/ml vs C 255.5 pg/ml; p < 0.05), PAR-2 (fIIPS 1807.9 pg/ml vs C 421.0 pg/ml; p < 0.05) and TGF-β1(fIIPS 283.0 pg/ml vs C 197.1 pg/ml; p < 0.01) in BALF in fIIPs versus C. The values of TNF-a in BALF did not differ significantly in fIIPs compared to controls. The ratios also showed differences in fIIPS and C: IL4Rα/TGF-β1 (fIIPS 6.19 vs C 0.68; p = 0.0143); TNF-α/IL4Rα (fIIPS 0.84 vs C 7.93; p = 0.043); TGF-β1/TNF-α (fIIPS 0.21 vs C 0.16; p = 0.0179) and protein/PAR-2 (fIIPS 0.06 vs C 0.28; p = 0.0033).
We found that PAR-2, TGF-β1 and IL-4Rα are significantly up-regulated in the BALF of fIIPs compared to controls, therefore we suppose they could become biomarkers of fibroproliferative healing.
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* Address correspondence to this author at the Department of Respiratory Medicine, Thomayer Hospital Prague, Videnska 800, 140 59 Prague 4, Czech Republic; Tel: + 42026108 2372; Fax: + 42026108 2206; E-mail: firstname.lastname@example.org