To investigate processing- and storage-dependent changes in D- and L-lactate concentration, blood samples
from eleven healthy Holstein calves were spiked with 3 mM D-lactic acid and 3 mM L-lactic acid immediately following
collection (time 0) or left untreated for comparison. Serum and plasma, respectively, were separated 0.5 hours following
collection or left in contact with blood cells, stored at 4°C and analyzed for D- and L-lactate concentration using
enzymatic assays at 1, 2, 4, 8, 12, 24, and 48 hours. Concentrations were compared to the 1 hour sample. D- and L-Lactate
concentrations in all separated samples were stable for up to 48 hours. When left in contact with cells, L-lactate
concentration in untreated and spiked serum and in spiked plasma, D-Lactate concentration in untreated serum, and total
lactate concentration in untreated serum increased significantly by 48 hours.