The Open Biotechnology Journal

ISSN: 1874-0707 ― Volume 15, 2021

In vivo Evaluation of the Repellency Effects of Nanoemulsion of Mentha piperita and Eucalyptus globulus Essential Oils against mosquitoes

Rahman Mohammadi1, Mehdi Khoobdel1, *, Ali A. Talebi2, Maryam Negahban3, Mohamad Norani4, Mohammad Moradi5, Omid Dehghan6
1 Health Research Center, Life Style Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran
2 Department of Entomology, Faculty of Agriculture, Tarbiat Modares University, P.O. Box 14115-336, Tehran, Iran
3 Iranian Research Institute of Plant Protection, Tehran, Iran
4 Department of Horticultural Science, Tarbiat Modares University, P.O. Box 14115-365, Tehran, Iran
5 Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
6 Department of Medical Entomology and Vector Control, Health Science Research Center, Faculty of Health, Mazandaran University of Medical Science, Sari, Iran



The present study aimed to prepare Nanoemulsions of Mentha piperita and Eucalyptus globulus essential oils and comparison of the repellant activity of them with normal essential oils and DEET in the field conditions.


To determine the protection and failure time of the essential oils and DEET in the field condition against natural population of night biting culicid mosquitoes, 4 human volunteers participated in night biting test. GC-MS was used to determine the essential oil components and the Dynamic Light Scattering device was used to measure droplet size and zeta potential.


The relative abundance of more common species captured in this study was 40.09% and 31.65% for Anopheles superpictus, and Culex pipiens, respectively. Based on the results, the protection time of nanoemulsions of M. piperita 50% against night biting mosquitoes was 4.96±0.21 h. Also, the protection for nanoemulsions essential oil 50% of E. globulus was 6.06±0.20 h. Comparison of the results showed that the protection time of nanoemulsions of M. piperita and E. globulus was significantly higher than of their normal essential oils (P˂0.01). Also, the protection time of DEET (as a gold standard) was significantly higher than of normal essential oil and nanoemulsions of M. piperita (P˂0.01), but there is no significant difference between DEET and nanoemulsions of E. globulus (P˃0.01).


Due to the safety and biocompatibility of the nanoessential oils, and also relatively adequate and acceptable protection time, nanoemulsions of E. globulus and probably M. piperita can be considered as good repellents. It is recommended to do more research on these nanoemulsion repellents, as they may be good alternatives to DEET.

Keywords: Culicidae, Essential oils, Mosquito repellent, Nanoemulsion, Night biting, DEET.

Article Information

Identifiers and Pagination:

Year: 2021
Volume: 15
Publisher Item Identifier (PII): BMS-TOBIOTJ-2020-21

Article History:

Electronic publication date: 11/1/2021
Collection year: 2021

© 2021 Mohammadi et al.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

* Address correspondence to this author at the Health Research Center, Life Style Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran; Tel/Fax: 0989126180432; E-mail:

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