Effect of Solvents on Phytochemicals Content and Antioxidant Activity of Ganoderma lucidum
Md. Moyen Uddin Pk1, Rabiul Islam Talukder1, Mohammad Khairul Islam Sarkar2, *, Tasnia Rahman2, Rumana Pervin3, Matiar Rahman3, Mst. Elina Akther Zenat4, Lima Akther5
1 Department of Biochemistry, Primeasia University, Dhaka, Bangladesh
2 School of Environmental Science and Management, Independent University Bangladesh (IUB), Dhaka, Bangladesh
3 Department of Biochemistry & Molecular Biology, University of Rajshahi, Bangladesh
4 Bangladesh Council of Scientific and Industrial Research (BCSIR), Dhaka, Bangladesh
5 Department of Chemistry, Comilla University, Bangladesh
The aim of this study was to assess the induction of solvents on the total phenolic and flavonoid content & also the antioxidant activity of Ganoderma lucidum extracts.
Materials & Methods:
In this study, two concentrations (100% and 75%) of diethyl ether, ethanol, butanol, chloroform, and acetone were used as extractants of Ganoderma lucidum. Total phenolic and flavonoid contents were measured by spectrophotometric methods and 1, 1-diphenyl-2-picrylhydrazyl (DPPH). Free radical scavenging assay was used for the investigation of antioxidant activity.
Results & Discussion:
Extractants significantly affected the % yield of extract, the quantity of phenol and flavonoids and antioxidant activity of Ganoderma lucidum mushroom. The highest extraction yield, around 38%, was achieved by 75% acetone, followed by 100% acetone (about 36%) and 75% chloroform (approximate 21%). Hydro-acetone extract exhibited the most significant antioxidative properties (EC50 value; 645.55 µg/mL) comprised of a higher total of phenolic content. In conclusion, the total phenolic content encouraged the antioxidative potential of Ganoderma lucidum mushroom.
These findings indicate that the selective extraction of Ganoderma lucidum shows significant biological activities.
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* Address correspondence to this author at the School of Environmental Science and Management, Independent University Bangladesh (IUB), Dhaka, Bangladesh Tel: +8801711230892; E-mail: firstname.lastname@example.org