Table 1: Staining with FITC-Dextran, PI or Annexin V-FITC apoptotic cells induced by a heat shock.
Cells*
% stained cells
FITC-Dextran
PI
nnexin V
Statistics (Student t test)
HeLa control
1.3±0.83
0.67±0.22
1.8±0.9
HeLa heated
79.6±5.5 %
18.2±1
42,6±3.8
p<0.001
PBMC control
1.42±0.86%
1.03±0.32
2±0.7
PBMC heated
70.3±6.2%
15±1.8%
38.9±4.5
p<0.001
*Cells were incubated at 43,5°C for 1 hour and at 37°C for 8 hours to induce apoptosis; then they were labelled with Annexin V-FITC, FITC-Dextran and PI, then analysed by flow cytometry. Control cells were unheated. The results represent the means ± S.D of 16 different samples, each tested twice