Table 1: Wet lab conditions of the analyzed image sets. Each image set was obtained in a different experiment and contains a varying number of fluorescence and brightfield images.
| Image set |
Activator |
Time after incubation |
Fluorescence dye |
| 1 |
None |
0 min |
None |
| 2 |
None |
0 min |
Fluo-4 |
| 3 |
LPA |
1 min |
Fluo-4 |
| 4 |
PMA |
15 min |
Fluo-4 |
| 5 |
A23187 |
15 min |
Fluo-4 |
| 6 |
A23187 |
30 min |
FITC |