Table 1: Materials. Major Materials used in the Assay are Listed in Table 1.

Name (Source) Description Catalog Number Note
ATP (Sigma-Aldrich) A7699
Poly-GT-biotin (Cisbio) Tyrosine kinase substrate 61GT0BLD 44KDa; Sequence: (GT)(4:1)
Poly-GAT-biotin (Cisbio) 61GATBLB 25KDa, Sequence: (GAT)(6:3:1)
TK substrate-biotin (Cisbio) 62TK0PEB 1.5KDa;*
PT66-K (Cisbio) Europium conjugated antibody specific for phosphorylated tyrosine residue 61Y20KLB
PY20-K (Cisbio) 61Y20KLB
TK antibody (Cisbio) 62TK0PEB
Streptavidin-XL665 (Cisbio) Detection reagent 610SAXLB
Staurosporine (Sigma-Aldrich) ATP-competitive inhibitor S4400
384-lv plate (Greiner) Black polystyrene 784067
RubyStar® (BMG) Microplate reader N/A
Syk enzyme Tyrosine kinase N/A 73kDa; specific activity = 96pmol/µg x min

* sequence provided by Cisbio to KinEASE TK users by Cisbio upon request.