Fig. (3) Pharmacological validation of Raf(V600E)/cSTKpep-3 and Raf(V600E)/MEK(WT) TR-FRET assays. IC₅₀ curves were determined for B-Raf inhibitor A in 384-well and 1536-well plates using double stable cell lines. Open circles: DMSO, solid squares: B-Raf inhibitor A.