Fig. (5). Case Study 2: AKT dependent cSTKpep-3 phosphorylation (A) HEK293 cells were transiently double transfected (1µg kinase DNA and/or 1µg substrate DNA in 6-well dish) as indicated and immunoblotted. (B) 384-well TR-FRET assay using a transient reverse transfection protocol. Wild-type Akt1(WT) or kinase-inactive Akt1(K179M) were combined with cSTKpep-3.