Fig. (6). Quantification of total- and phospho-cSTKpep levels can reveal compound interference. The importance of short compound incubation (A) Combinations of α-FLAG with α-p-STK or α-Myc antibodies were used to quantify either cSTKpep-phosphorylation or total cellular cSTKpep-3 expression by TR-FRET. (B) and (C) Transient 384-well AKT/cSTKpep-3 TR-FRET assay. Cells were treated with staurosporine for 2h (B) or 48h (C). Open circles: phospho-cSTKpep-3; Solid circles: total-cSTKpep-3.