Fig. (8). Induction of CamK2δ activity Transient transfection reactions were performed in 384-well plates as indicated. HEK 293 were reverse transfected with 50ng CamK2δ and 50ng (black bars) or 25ng (gray bars) cSTKpep-1. CamK2δ activity was induced through 5min ionomycin treatment (1µM) and p-cSTKpep-1 was quantified by TR-FRET.