Table 2: An Emerging Cellular Ser/Thr-Kinase Assay Panel. Confirmed hits obtained from cSTKpep cDNA screens. fold activation was calculated as TR-FRET signal with kinase and cSTKpep cotransfection divided by cSTKpep alone.
| Gene Name |
Accession number |
Peptide Substrate |
Fold Activation |
Kinase Family |
| Biochemical |
Cellular |
| B-Raf(V600E) |
NM_004333 |
|
3 |
9.0 |
TKL |
| Cot |
NM_005204 |
|
3 |
8.5 |
STE |
| PKA |
BC054834 |
2 |
2 |
8.5 |
AGC |
| Akt1 |
NM_005163.1 |
3 |
3 |
9.9 |
AGC |
| Akt2 |
NM_001626 |
3 |
3 |
4.0 |
AGC |
| B-RAF(WT) |
NM_004333 |
|
3 |
5.0 |
TKL |
| CHEK2 |
NM_007194.2 |
1 |
3 |
9.0 |
CMGC |
| MEKK3 |
NM_002401.3 |
|
3 |
8.9 |
STE |
| MLK3 |
NM_002419.2 |
|
3 |
5.3 |
TKL |
| Pim-2 |
BC018111 |
3 |
3 |
7.4 |
CAMK |
| PKD1 |
NM_002742.1 |
|
3 |
9.1 |
CAMK |
| PKD2 |
BC095949 |
1 |
3 |
5.9 |
CAMK |
| PRKX |
NM_005044.1 |
2 |
2 |
9.0 |
AGC |
| Aurora-A |
BC001280 |
2 |
2 |
4.3 |
AGC |
| BRSK2 |
NM_003957.1 |
1 |
3 |
2.2 |
CAMK |
| CAMK2*
|
BC032784 |
1 |
1 |
2.4 |
CAMK |
| MEK1-CA |
NM_002755 |
|
3 |
3.4 |
STE |
| MKK3 |
BC032478 |
|
3 |
3.0 |
STE |
| MKK6 |
NM_002758.2 |
|
3 |
4.9 |
STE |
| NEK6 |
NM_014397.3 |
3 |
3 |
2.0 |
CAMK |
| NEK7 |
NM_133494.1 |
3 |
3 |
4.0 |
CAMK |
| PAK4 |
BC048238 |
2 |
3 |
2.9 |
STE |