Comparison on Functional Assays for Gq-Coupled GPCRs by Measuring Inositol Monophospate-1 and Intracellular Calcium in 1536-Well Plate Format

Fig. (6) DMSO plate tests in a M₁-CHO cell line for the intracellular calcium and IP-One assays. (a) Scatter plot of the results from IP-One assay. All wells were stimulated the EC₈₀ amount of carbachol (4 μM). Column 2 was received 50 μM atropine (IC₁₀₀). (b) Scatter plot of the results from intracellular calcium assay. All wells in this plate were stimulated with 0.1 μM carbachol (in EC₈₀). Column 1 was treated with 50 μM atropine (IC₁₀₀) and column 2 was added with the EC₁₀₀ amount of carbachol.