Fig. (5) Hematoxylin and Eosin (H&E) stained of ventricular area. Histological sections stained with hematoxylin and eosin of hearts from sham, vehicle, and Vit D treated mice after 72-hour post I/R. In sham (A), myocardial tissue structurally clear, while the myocardial cells of I/R-mice and vehicle treated (B, C) had edema, inflammatory cells infiltration hemorrhage, and necrosis. While, vit D treated mice (D) attenuated cardiac injury. According to the Zingarelli scoring system; score 0 for sham mice group; 4 scoring to I/R alone and vehicle group; and score 2 (moderate) for vit D treated group (Fig. 5e).