Fig. (3). Evaluation of TEV Protease Recognition Sequence P₁' Specificity Cleavage dependent activation of CP₂₃₄-Luc/ENLYFQX fusion proteins. Cell-free translation reactions were diluted 1:1 in 2X TEV protease buffer, incubated at 30°C for 30 minutes ± 10 U TEV protease and luminescence was measured from 5 µL aliquots. The X-axis is the amino acid at the P₁' position (X) of the CP₂₃₄-Luc/ENLYFQX fusion protein. The Y-axis is the fold activation of the CP₂₃₄-Luc/ENLYFQX fusion proteins calculated by dividing the mean TEV digested values by the mean undigested values (no TEV digest), N = 3. Error bars are the standard deviation of the mean.