Fig. (5). Representative Data from Plate 1 of the LOPAC¹²⁸⁰ Library Screen The LOPAC¹²⁸⁰ library was screened for TEV protease inhibitors using the CP₂₃₄-Luc/ENLYFQC assay. Shown here are the data from plate 1 (N = 320 compounds). There were three hits () based on greater than 60% TEV protease inhibition, blue line (= 345,311 RLU). The CP₂₃₄-Luc/42AA assay was also performed to detect potential non-specific affectors of the CP₂₃₄-Luc assay and the results, which are overlaid on the graph in gray, indicate that two compounds non-specifically affected the assay. () = CP₂₃₄-Luc/42AA activity in DMSO; () = CP₂₃₄-Luc/42AA activity with compound library; () = CP₂₃₄-Luc/ENLYFQC activity minus TEV enzyme in DMSO; () = CP₂₃₄-Luc/ENLYFQC activity plus TEV enzyme in DMSO, and () = CP₂₃₄-Luc/ENLYFQC activity plus TEV protease with compound library. Controls are plotted as the mean of N = 64 (CP₂₃₄-Luc/42AA in DMSO) or 32 (CP₂₃₄-Luc/ENLYFQC ± TEV protease in DMSO). Error bars are the standard deviation of the mean.