Table 2: Oligonucleotide Pairs for the Interrogation of TEV Protease Recognition Sequence P₁' Position (ENLYFQX)

X =	Forward primer sequence (5' - 3')	Reverse primer sequence (5' - 3')
A	CTAGCGAGAACCTGTACTTCCAGGCCG	GATCCGGCCTGGAAGTACAGGTTCTCG
C	CTAGCGAGAACCTGTACTTCCAGTGCG	GATCCGCACTGGAAGTACAGGTTCTCG
D	CTAGCGAGAACCTGTACTTCCAGGACG	GATCCGTCCTGGAAGTACAGGTTCTCG
E	CTAGCGAGAACCTGTACTTCCAGGAGG	GATCCCTCCTGGAAGTACAGGTTCTCG
F	CTAGCGAGAACCTGTACTTCCAGTTCG	GATCCGAACTGGAAGTACAGGTTCTCG
G	CTAGCGAGAACCTGTACTTCCAGGGCG	GATCCGCCCTGGAAGTACAGGTTCTCG
H	CTAGCGAGAACCTGTACTTCCAGCACG	GATCCGTGCTGGAAGTACAGGTTCTCG
I	CTAGCGAGAACCTGTACTTCCAGATCG	GATCCGATCTGGAAGTACAGGTTCTCG
K	CTAGCGAGAACCTGTACTTCCAGAAGG	GATCCCTTCTGGAAGTACAGGTTCTCG
L	CTAGCGAGAACCTGTACTTCCAGCTGG	GATCCCAGCTGGAAGTACAGGTTCTCG
M	CTAGCGAGAACCTGTACTTCCAGATGG	GATCCCATCTGGAAGTACAGGTTCTCG
N	CTAGCGAGAACCTGTACTTCCAGAACG	GATCCGTTCTGGAAGTACAGGTTCTCG
P	CTAGCGAGAACCTGTACTTCCAGCCAG	GATCCTGGCTGGAAGTACAGGTTCTCG
Q	CTAGCGAGAACCTGTACTTCCAGCAGG	GATCCCTGCTGGAAGTACAGGTTCTCG
R	CTAGCGAGAACCTGTACTTCCAGCGCG	GATCCGCGCTGGAAGTACAGGTTCTCG
S	CTAGCGAGAACCTGTACTTCCAGAGCG	GATCCGCTCTGGAAGTACAGGTTCTCG
T	CTAGCGAGAACCTGTACTTCCAGACCG	GATCCGGTCTGGAAGTACAGGTTCTCG
V	CTAGCGAGAACCTGTACTTCCAGGTGG	GATCCCACCTGGAAGTACAGGTTCTCG
W	CTAGCGAGAACCTGTACTTCCAGTGGG	GATCCCCACTGGAAGTACAGGTTCTCG
Y	CTAGCGAGAACCTGTACTTCCAGTACG	GATCCGTACTGGAAGTACAGGTTCTCG