Fig (3) Effects of various HSF-specific RNAi oligos on 17-AAG and heat shock-induced beta-lactamase reporter activity. (A) Cells were incubated with the indicated RNAi oligos for 40 hrs, followed by a medium change to Assay Medium. Cells were then stimulated with 400 nM 17-AAG for 6 hours before the beta-lactamase assay was performed as described in Methods. (B) Cells were incubated with the indicated RNAi oligos for 40 hrs, followed by heat shock at 42°C for 1 hour and a 5-hour recovery at 37° C before the beta-lactamase assay was performed as described in Methods (n=3 for each data point). Controls were as follows: nontransfected and un-stimulated cells (NT unstim), nontransfected and stimulated cells (NT stim), mock transfected (mock), Stealth^TM RNAi Negative Control Med GC (MedGC), and beta-lactamase positive control RNAi duplex (b-lac).