Table 1: Protocol for HSE-bla Assay in 384-well Plate Format

Step	Parameter	Value	Description
1	HSE-bla Cells	36 µL	8,000 cells/well
2	Incubation	16-18 hrs	37°C
3	Compound	4 µL	17-AAG
4	Incubation	5 hrs	37°C
5	Substrate	8 µL	CCF4-AM
6	Incubation	2 hrs	Room Temp.
7	Detection	fluorescence	Tecan Safire2