Table 2: Protocol for HSE-bla Assay in 1536-well Plate Format

Step	Parameter	Value	Description
1	HSE-bla Cells	5 µL	1500 cells/well
2	Incubation	16-18 hrs	37°C
3	Compound	23 nL	17-AAG or DMSO
4	Incubation	6 hrs	37°C
5	Substrate	1 µL	CCF4-AM
6	Incubation	2 hrs	Room Temp.
7	Detection	fluorescence	Envision