Table 1:
Resazurin-Reduction Assay Z’-Factor
Cells/Well
Mean Fluorescence after 4hr at 37°C
Standard Deviation
Z’-Factor
0
455.28
5.76
500
1074.58
34.67
0.8
2000
3056.88
125.37
0.85
2500
3602.1
132.98
0.87
CellTiter-Blue® (Promega Corporation) was added and incubated with L929 cells dispensed in 384 well plates. n = 96 for each cell concentration.