In Vitro Viability and Cytotoxicity Testing and Same-Well Multi-Parametric Combinations for High Throughput Screening

Table 2: Viability and Cytotoxicity Assay Reagents for HTS

Assay Type	Reagent Name	Biomarker	Detection Method^{^¥}	Reagent Incubation Period	Sensitivity^*(cells/well)	Multiplex Options#
Viability	CellTiter Blue^®	Reductase(s)	Fluorescence	2-4 hrs	50	Yes^†
	CellTiter-Fluor^™	Protease(s)	Fluorescence	30 min	10-50	Yes
	CellTiter-Glo^®	ATP	Luminescence	10 min	10	Yes^†
Cytotoxicity	CytoTox-ONE^™	LDH	Fluorescence	10 min	~200	Yes^†
	CytoTox-Fluor^™	Protease(s)	Fluorescence	30 min	10-50	Yes
	CytoTox-Glo^™	Protease(s)	Luminescence	15 min	10	Yes^†
Viability and Cytotoxicity	MultiTox-Fluor	Protease(s)	Fluorescence/Fluorescence	30 min	10-50/10-50	Yes
	MultiTox-Glo	Protease(s)	Fluorescence/Luminescence	30 min/15min	10-50/10	Yes
	CytoTox-Glo^™With (2^nd step lysis)	Protease(s)	Luminescence/Luminescence	15 min/15 min	10/10	Yes^†

^¥ Detection method for combination viability and cytotoxicity assays is denoted as viability format/cytotoxicity format.

^* Sensitivity was determined by limiting dilution series in the presence of 10% fetal bovine serum and is dependent upon cell type and instrumentation.

^† Options exist, but may be assay sequence-dependent or require slight deviation from standard protocol.