Fig. (1) Western blot analysis of total phosphorylation of Met. Samples of 5 µg and 50 ng of unphosphorylated (right band) and phosphorylated (left band) c-Met, respectively were resolved by SDS-PAGE under reducing conditions (upper panel). Phosphorylation content was determined using pan anti-phosphotyrosine antibodies (middle panel). Phosphorylation of Y1234 and Y1235 was detected by anti-phospho c-Met (pY1234/pY 1235 (lower panel).